Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Molecular Biology
A Guide to the NGS Workflow
NGS Library Preparation and Sample Prep
KAPA Pure Beads
KAPA Pure Beads from Roche
Be the first to write a review!
Citations:
(235)
Supplier Page
Supplier Page from
Roche for
KAPA Pure Beads
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
KAPA Pure Beads
Company
Roche
Price
Supplier Page
View Company Product Page
Catalog Number
07983271001
Quantity
5 mL
Add to Compare List
Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (235)
(21)
SARS-CoV-2 Recombination and Coinfection Events Identified in Clinical Samples in Russia
Viruses
July 30, 2023
Ekaterina N. Chernyaeva, Andrey A. Ayginin, Alexey V. Kosenkov, Svetlana V. Romanova, Anastasia V. Tsypkina, Andrey R. Luparev, Ivan F. Stetsenko, Natalia I. Gnusareva, Alina D. Matsvay, Yulia A. Savochkina,
et al.
agarose gel electrophoresis
(2%). Amplicon
purification
was performed using surface-modified magnetic particles from
KAPA Pure Beads
(
KAPA Biosystems
, Wilmington, MA, USA). Measurement of the concentration of nucleic acids in the purified
(22)
Variations in the Physical Properties and Microbial Community of Dairy Cow Manure—Implications for Testing and Efficacy of Footbathing Products
Animals (Basel)
July 22, 2023
Maeve A. Palmer, Martin J. Garland, Linda D. Stewart, Sarah J. Helyar, Niamh E. O’Connell
for 30 s, followed by a final extension step at 72 °C for 5 min. The
PCR
products were cleaned using
KAPA Pure Beads
(
Roche
, Wilmington, MA, USA), and the size of
PCR
products were identified on a Fragment Analyzer (Advanced
(23)
Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains
Scientific Reports
July 5, 2023
Ana Moya-Beltrán, Martin Gajdosik, Camila Rojas-Villalobos, Simón Beard, Martin Mandl, Danitza Silva-García, D. Barrie Johnson, Pablo Ramirez, Raquel Quatrini, Jiri Kucera
PureYield™ Plasmid Miniprep System
(
Promega
). Extracted genomic DNA was purified on magnetic beads (
KAPA Pure Beads
,
Roche
) according to the standard protocol. The DNA concentration was measured using a
Qubit ™ dsDNA HS Assay kit
(24)
A type II protein arginine methyltransferase regulates merozoite invasion in Plasmodium falciparum
Communications Biology
June 22, 2023
Amuza Byaruhanga Lucky, Chengqi Wang, Min Liu, Xiaoying Liang, Hui Min, Qi Fan, Faiza Amber Siddiqui, Swamy Rakesh Adapa, Xiaolian Li, Rays H. Y. Jiang,
et al.
the manufacturer’s recommendations. Amplified DNA libraries were captured by incubating with 1.3 ×
Kapa pure beads
(
Roche
#KK8001) according to the manufacturer’s recommendations, and 150 bp paired-end
sequencing
was
(25)
Variation of growth and transcriptome responses to arbuscular mycorrhizal symbiosis in different foxtail millet lines
Botanical Studies
June 16, 2023
Ou-Chi Chang, Wei-Yi Lin
KAPA mRNA HyperPrep Kit
(
Roche
, Switzerland) and 300–400 bp fragments containing adaptors were sorted using a
KAPA Pure Beads
system (
Roche
) following the manufacture’s instruction.
Sequencing
was performed on a NovaSeq 6000 platform
(26)
Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
Frontiers in Microbiology
June 12, 2023
Giovanni Bacci, Sara Fratini, Niccolò Meriggi, Christine L. Y. Cheng, Ka Hei Ng, Massimo Pindo, Alessio Iannucci, Alessio Mengoni, Duccio Cavalieri, Stefano Cannicci
specification. All
PCR
products were checked through
electrophoresis
on 1.5% agarose gel and then purified using
KAPA Pure Beads
(
Roche
) following the manufacturer’s instructions. To apply the Illumina adapters
sequencing
indexing
(27)
New insight into the bark beetle ips typographus bacteriome reveals unexplored diversity potentially beneficial to the host
Environmental Microbiome
June 9, 2023
Ezequiel Peral-Aranega, Zaki Saati-Santamaría, Miguel Ayuso-Calles, Martin Kostovčík, Tereza Veselská, Karel Švec, Raúl Rivas, Miroslav Kolařik, Paula García-Fraile
(Kapa Biosystems, Massachusetts, USA). Amplicon’s size selection of the final libraries was done by
KAPA Pure Beads
(
Kapa Biosystems
) and its effectiveness was checked on 1% agarose gel (SeaKem® LE Agarose, Lonza Group
(28)
Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression
Immunogenetics
June 1, 2023
Vitor R. C. Aguiar, Erick C. Castelli, Richard M. Single, Arman Bashirova, Veron Ramsuran, Smita Kulkarni, Danillo G. Augusto, Maureen P. Martin, Maria Gutierrez-Arcelus, Mary Carrington,
et al.
hybridization
,
RNase H
was used to enzymatically degrade rRNA. Cleanup and DNase digestion was performed using
Kapa Pure Beads
and DNase according to
Kapa
protocol. Fragmentation,
cDNA synthesis
, and
library construction
rRNA
(29)
Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology
bioRxiv
May 20, 2023
Thomas Goralski, Lindsay Meyerdirk, Libby Breton, Laura Brasseur, Kevin Kurgat, Daniella DeWeerd, Lisa Turner, Katelyn Becker, Marie Adams, Daniel Newhouse,
et al.
PCR master mix, 4 μL primer from the correct wells, and 4 μL resuspended DSP aspirate. KAPA beads (
KAPA Pure beads
,
Roche
Cat#
07983298001
) were warmed to room temperature for 30 minutes. Libraries were pooled and KAPA
(30)
Comparison of Immune-Related Gene Expression in Two Chicken Breeds Following Infectious Bronchitis Virus Vaccination
Animals (Basel)
May 15, 2023
Schwann Chuwatthanakhajorn, Chi-Sheng Chang, Kannan Ganapathy, Pin-Chi Tang, Chih-Feng Chen
To select cDNA fragments of 300–400 base pairs (bp), the library fragments were purified using the
KAPA Pure Beads
system (
KAPA Biosystems
, Roche, Basel, Switzerland). The library carrying the appropriate adapter sequences
(31)
Chloroquine resistance evolution in Plasmodium falciparum is mediated by the putative amino acid transporter AAT1
Nature Microbiology
May 11, 2023
Alfred Amambua-Ngwa, Katrina A. Button-Simons, Xue Li, Sudhir Kumar, Katelyn Vendrely Brenneman, Marco Ferrari, Lisa A. Checkley, Meseret T. Haile, Douglas A. Shoue, Marina McDew-White,
et al.
(Invitrogen). For samples with <50 ng DNA obtained, we performed WGA52. WGA products were cleaned with
KAPA Pure Beads
(
Roche
Molecular Systems) at a
1:1
ratio. We prepared
sequencing
libraries using 50–100 ng DNA or WGA
(32)
Mouse B2 SINE elements function as IFN-inducible enhancers
eLife
May 9, 2023
Isabella Horton, Conor J Kelly, Adam Dziulko, David M Simpson, Edward B Chuong
HSD5000 (Agilent) before proceeding to library preparation. Pulldowns were concentrated and pooled using
KAPA Pure Beads
(
Roche
). The final pooled libraries were quantities with TapeStation 4200 HSD5000 and sequences on an
(33)
Spatial and temporal dynamics at an actively silicifying hydrothermal system
Frontiers in Microbiology
May 3, 2023
Kalen L. Rasmussen, Blake W. Stamps, Gary F. Vanzin, Shannon M. Ulrich, John R. Spear
1:30
min), a final extension of 68°C for 5 min, and a final 4°C hold. The amplicons were cleaned using
KAPA Pure Beads
(
KAPA Biosystems
Inc., Wilmington, MA, United States) at a final concentration of 0.8X v/v to remove
(34)
Enhancer co-amplification and hijacking promote oncogene expression in liposarcoma
Cancer Research
May 2, 2023
Tingting Liu, Juan Wang, Hongbo Yang, Qiushi Jin, Xiaotao Wang, Yihao Fu, Yu Luan, Qixuan Wang, Mark W. Youngblood, Xinyan Lu,
et al.
according to the manufacturer's protocol and amplified eight cycles by
PCR
. After
purification
by 0.85×
KAPA pure beads
, the libraries were sequenced on Hi-seq 2500 or NovaSeq platform with 2 × 100 bp setting.
Whole-genome sequencing
(35)
A Higher Abundance of Actinomyces spp. in the Gut Is Associated with Spontaneous Preterm Birth
Microorganisms
April 29, 2023
Hong-Ren Yu, Ching-Chang Tsai, Julie Y. H. Chan, Wei-Chia Lee, Kay L. H. Wu, You-Lin Tain, Te-Yao Hsu, Hsin-Hsin Cheng, Hsin-Chun Huang, Cheng-Hsieh Huang,
et al.
using a Fragment Analyzer system (Agilent Technologies Inc.). The amplified DNA was then purified using
KAPA Pure Beads
(
Roche
) according to the manufacturer’s protocol. Library index
PCR
was performed using an Illumina
(36)
Evaluation of Library Preparation Workflows and Applications to Different Sample Types Using the PowerSeq® 46GY System with Massively Parallel Sequencing
Genes (Basel)
April 26, 2023
Kyleen Elwick, Patrick Rydzak, James M. Robertson
finally, a 1 min final extension at 72 °C. Post
amplification
required a 1× bead-based cleanup using 50 µL
KAPA Pure Beads
(KPBs,
Roche
Sequencing
Solutions). The plate was incubated for 10 min at room temperature and then
(37)
Characterization of the Bacterial Communities Inhabiting Tropical Propolis of Puerto Rico
Microorganisms
April 26, 2023
Ana E. Pérez Matos, Giovanni Bacci, Luigimaria Borruso, Maria Landolfi, Dominique Petrocchi, Sonia Renzi, Brunella Perito
55 °C (30 s), 72 °C (30 s), then a final extension of 72 °C (5 min). Libraries were purified using
KAPA Pure beads
(
Roche Diagnostics
SpA, Monza, Italy), and then dual indices from the Illumina Nextera XT Index Kit
(38)
Unmasking the tissue-resident eukaryotic DNA virome in humans
Nucleic Acids Research
April 24, 2023
Lari Pyöriä, Diogo Pratas, Mari Toppinen, Klaus Hedman, Antti Sajantila, Maria F Perdomo
viral enrichment, the libraries were amplified 3 × 13–25 cycles. The
clean-up
steps were performed with
KAPA Pure Beads
(
Roche
). Negative controls (PCR-grade water) were included in the library preparation, enrichment, and
(39)
Lung-gut axis of microbiome alterations following co-exposure to ultrafine carbon black and ozone
Particle and Fibre Toxicology
April 21, 2023
Md Habibul Hasan Mazumder, Jasleen Gandhi, Nairrita Majumder, Lei Wang, Robert Ian Cumming, Sydney Stradtman, Murugesan Velayutham, Quincy A. Hathaway, Jonathan Shannahan, Gangqing Hu,
et al.
and 72 °C for 30 s, and 72 °C for 5 min for the final extension. The
PCR
products were purified using
KAPA pure beads
(
KAPA
Byosystems, Wilmington, MA, USA) using a
Magnetic Stand-96
(
Thermo Fisher Scientific
). The
PCR
(40)
Population dynamics and drug resistance mutations in Plasmodium falciparum on the Bijagós Archipelago, Guinea-Bissau
Scientific Reports
April 18, 2023
Sophie Moss, Emilia Mańko, Hristina Vasileva, Eunice Teixeira Da Silva, Adriana Goncalves, Ashley Osborne, Jody Phelan, Amabelia Rodrigues, Paulo Djata, Umberto D’Alessandro,
et al.
samples amplified.
Whole genome sequencing
The SWGA product from these samples was purified using
KAPA pure beads
(
Roche
). Beads were equilibrated to room temperature and mixed with SWGA product at a
1:1
volume ratio.
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
KAPA Pure Beads from Roche
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
Back To Top